What is the different between tissue-specific knockout mouse and an inducible knockout mouse
A tissue-specific knockout mouse and an inducible knockout mouse are two different types of genetic mouse models that are specifically engineered to study the effects of gene deletion or inactivation in living organisms. Let me explain the differences between them:
1. Tissue-specific knockout mouse:
- In a tissue-specific knockout mouse, a particular gene is deleted or inactivated only in specific tissues or cell types.
- This is achieved by using a genetic technique called Cre-Lox recombination, where a specific DNA recombinase enzyme (Cre) is used to delete the target gene in a tissue-specific manner.
- By selectively deleting a gene in a specific tissue or organ, researchers can observe the functional consequences and study the specific role of that gene in the targeted tissue.
2. Inducible knockout mouse:
- In an inducible knockout mouse, the deletion or inactivation of a gene can be controlled or induced at a specific time point by researchers.
- This is usually accomplished by using another genetic technique called the tetracycline-controlled transactivator system (Tet-On/Tet-Off system) or the tamoxifen-inducible Cre recombinase system.
- These systems allow researchers to control the expression or inactivation of the target gene in response to specific cues such as the presence or absence of a specific chemical (e.g., tetracycline or tamoxifen).
The main difference between these two types of knockout mice lies in the spatial and temporal control of gene deletion. Tissue-specific knockout mice focus on deleting or inactivating a gene only in specific tissues, while inducible knockout mice allow researchers to control the time at which the gene is deleted or inactivated.
Both types of knockout mouse models have their advantages and provide valuable insights into gene function and its effects on specific tissues or organs. The choice between tissue-specific or inducible knockout depends on the research question and the desired level of control over gene deletion or inactivation.