You have a stock solution of 3.5M glucose. You need a working solution of 0.5M glucose in a volume of 400ml. Explain how you make the solution. Also what is the concentration of the dilution solution?
To prepare a working solution of 0.5M glucose from the stock solution of 3.5M glucose, you will need to perform a dilution. Here's how you can make the solution:
1. Determine the volume of the stock solution required for the dilution. You can use the formula C1V1 = C2V2, where C1 = concentration of the stock solution, V1 = volume of the stock solution, C2 = desired concentration of the working solution, and V2 = final volume of the working solution. Plugging in the values, we have: (3.5M)(V1) = (0.5M)(400ml).
2. Solve for V1: V1 = (0.5M * 400ml) / 3.5M. Evaluating this expression gives V1 = 57.14 ml (rounded to 2 decimal places).
3. Measure 57.14ml of the 3.5M glucose stock solution using a pipette or other measuring device.
4. Transfer the measured volume of the stock solution into a larger container, such as a beaker or flask.
5. Add diluent (solvent) to reach a final volume of 400ml. Diluent can be distilled water or any suitable solvent that will not react or interfere with the glucose solution.
6. Thoroughly mix the contents of the container to ensure a homogeneous working solution.
The concentration of the dilution solution, or the diluent, is technically 0M since it does not contain any solute (glucose).
To prepare a working solution of 0.5M glucose from a stock solution of 3.5M glucose, you will need to dilute the stock solution with a dilution solution (usually water or another suitable solvent). Here's a step-by-step explanation of how to make the solution:
1. Determine the final volume:
- The working solution needs to have a volume of 400ml.
2. Calculate the required amount of glucose:
- Since the final concentration required is 0.5M, you need to calculate the amount of glucose needed to achieve this concentration.
- Use the formula: C1V1 = C2V2
- Where C1 is the initial concentration (3.5M), V1 is the initial volume (unknown), C2 is the final concentration (0.5M), and V2 is the final volume (400ml).
- Rearrange the formula to solve for V1: V1 = (C2V2) / C1
- Plug in the values: V1 = (0.5M * 400ml) / 3.5M
- Calculate: V1 = 57.14ml
3. Measure the required volume of stock solution:
- Using a pipette or other precise measuring device, measure and transfer 57.14ml of the 3.5M glucose stock solution.
4. Add the dilution solution:
- Fill a container (such as a beaker or flask) with the desired final volume of 400ml.
- Add the measured 57.14ml of the 3.5M glucose stock solution to the container.
- Carefully fill the rest of the container with the dilution solution (water) until the total volume reaches 400ml.
Now, let's calculate the concentration of the dilution solution:
- The concentration of the dilution solution is the same as the final concentration of the working solution (0.5M). This means that the dilution solution should be used without any dilution.
- Therefore, the concentration of the dilution solution is 0.5M.
Remember to appropriately label your containers and ensure accurate measurements during the preparation process.