If catalase enzyme was placed in a test tube and heated to 100 degrees C, what results would you expect from a glowing splint test?
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Would it be: nothing, because at 100 degrees the enzymes would be denatured and would therefore not cause a reaction to occur? And if no reaction occurred, there would be no oxygen for the glowing splint test.
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When catalase enzyme is heated to 100 degrees Celsius, it will no longer be functional. As a result, the enzyme will be denatured, losing its ability to catalyze the breakdown of hydrogen peroxide into water and oxygen gas.
This means that if you were to perform a glowing splint test, where a glowing splint is inserted into a test tube containing the enzyme, you would not observe any significant reaction. Normally, when catalase is active, it catalyzes the decomposition of hydrogen peroxide, releasing oxygen gas. This oxygen gas would then relight a glowing splint, causing it to burst into flame.
However, since the catalase enzyme has been denatured due to the high temperature, it will not be able to break down hydrogen peroxide effectively anymore. Consequently, there will be no significant release of oxygen gas, and hence no relighting or bursting into flame of the glowing splint.
In summary, when catalase enzyme is heated to 100 degrees Celsius, the results of the glowing splint test would be negative, as there would be no observable reaction indicating the presence of active catalase enzyme.