if i use 30 ul from a total volume of 600ul to get 40ug/ml. So to get the total ug only, do i take 40ug/ml*.030ml*(600ul/30ul)=total ug

To calculate the total amount in micrograms (ug), you would multiply the concentration (40ug/ml) by the volume (30ul) and then adjust for the total volume (600ul) and the volume used (30ul).

Here is the step-by-step calculation:

1. Multiply the concentration (40ug/ml) by the volume used (30ul):
40ug/ml * 30ul = 1200ug

2. Adjust for the total volume:
Multiply the result from step 1 (1200ug) by the ratio of the total volume to the volume used:
1200ug * (600ul / 30ul) = 1200ug * 20 = 24000ug

Therefore, the total amount in micrograms (ug) would be 24,000ug.