if i use 30 ul from a total volume of 600ul to get 40ug/ml. So to get the total ug only, do i take 40ug/ml*.030ml*(600ul/30ul)=total ug
To calculate the total micrograms (μg), you can use the following formula:
Total μg = concentration (μg/ml) * volume (ml) * dilution factor
In this case, you used 30 μl from a total volume of 600 μl to achieve a concentration of 40 μg/ml. The dilution factor is calculated by dividing the final volume used (30 μl) by the initial volume (600 μl), which gives you a dilution factor of 20.
Now, let's calculate the total micrograms:
Total μg = 40 μg/ml * 0.030 ml * (600 μl/30 μl)
= 40 μg/ml * 0.030 ml * 20
= 24 μg
Therefore, the total micrograms obtained would be 24 μg.