If the base sequence along a segment of DNA were TCGTA,what would be the antisense oligonucleotide synthesised from this sequence?
To determine the antisense oligonucleotide synthesized from the given DNA base sequence TCGTA, we need to understand the complementary base pairing rules in DNA.
In DNA, adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). The antisense strand is the complementary strand to the sense or template strand.
Given the sequence TCGTA, we can determine the complementary bases:
T pairs with A
C pairs with G
G pairs with C
T pairs with A
A pairs with T
So the complementary sequence of TCGTA is AGCAT.
To synthesize the antisense oligonucleotide, we would use the complementary sequence as the template. Therefore, the antisense oligonucleotide synthesized from the given DNA sequence TCGTA is AGCAT.
To find the antisense oligonucleotide synthesized from the base sequence TCGTA, we need to complement each base with its corresponding base.
The base pairing rules are as follows:
- Adenine (A) pairs with Thymine (T)
- Thymine (T) pairs with Adenine (A)
- Guanine (G) pairs with Cytosine (C)
- Cytosine (C) pairs with Guanine (G)
So, to find the antisense sequence, we would have the following complement base pairs:
T (Thymine) pairs with A (Adenine)
C (Cytosine) pairs with G (Guanine)
G (Guanine) pairs with C (Cytosine)
T (Thymine) pairs with A (Adenine)
A (Adenine) pairs with T (Thymine)
The antisense oligonucleotide synthesized from the base sequence TCGTA would be AGCAU.