Sometimes, certain enzymes are associated with a particular organelle of the cell. Many times scientists have had to use ________________ centrifugation to separate different constituents of the cell. Following this procedure, you aliquot the fractions into separate test tubes, each correlating to a particular layer (or fraction) of your centrifugation end product. One way to help isolate an enzyme from among all the fractions collected is to assay for enzyme ___________. This will help delineate which fraction the enzyme is in, and even some of its properties. (Points: 4)

a. differential, activity
b. micro, presence
c. micor, products
d. swing-bucket, activity

Blank 1:

http://en.wikipedia.org/wiki/Differential_centrifugation

Blank 2:
http://en.wikipedia.org/wiki/Enzyme_activity

Blank 1: a. differential

To separate different constituents of the cell, scientists often use differential centrifugation. This technique involves spinning the cell suspension at different speeds, which results in the formation of distinct layers or fractions based on their density. By adjusting the centrifugal force and time, scientists can separate organelles and other cellular components into different fractions.

You can learn more about differential centrifugation on the Wikipedia page [Differential Centrifugation](http://en.wikipedia.org/wiki/Differential_centrifugation).

Blank 2: b. activity

To isolate an enzyme from the different fractions obtained through differential centrifugation, one way is to assay for enzyme activity. Enzyme activity refers to the ability of an enzyme to catalyze a specific reaction. By performing an assay to measure the enzyme activity in each fraction, you can determine which fraction contains the enzyme of interest.

Assaying for enzyme activity allows scientists to not only identify the fraction that contains the enzyme but also gain insights into its properties and behavior.

You can find more information about enzyme activity on the Wikipedia page [Enzyme Activity](http://en.wikipedia.org/wiki/Enzyme_activity).