a solution has gluten concentration of 2000ug/mL at 150uL. if 2uLof that solution is taken out what is the quantity of gluten in ppm?
I'm confused. If it has a concn of 2000 ug/mL, what's with the 150 uL. Are you diluting the 2000 ug/mL soln by taking 2 uL an diluting to 150 uL?
no i think we don't need to use 150ul.. can u solve it without using 150ul?
2000 ug/mL = 2000 ppm whether you have 1 mL or 10 L
To calculate the quantity of gluten in ppm (parts per million) after taking out 2uL of a solution, we need to follow these steps:
Step 1: Convert the concentration from ug/mL to ug/uL.
Given that the concentration of gluten is 2000 ug/mL, we can assume that 1 mL is equal to 1000 uL.
So, 2000 ug/mL can be converted to:
(2000 ug/mL) * (1 mL / 1000 uL) = 2 ug/uL
Step 2: Calculate the quantity of gluten in the remaining solution.
The initial solution volume is 150 uL, and 2 uL was taken out.
Therefore, the remaining volume is:
150 uL - 2 uL = 148 uL
Step 3: Calculate the quantity of gluten in the remaining solution in ug.
To find the quantity of gluten in the remaining solution, we multiply the concentration (2 ug/uL) by the volume (148 uL):
2 ug/uL * 148 uL = 296 ug
Step 4: Convert the quantity of gluten from ug to ppm.
To convert the quantity of gluten from ug to ppm, we need to know the total volume of the solution, in this case:
150 uL
Now we can calculate the concentration in ppm:
(296 ug / 150 uL) * (1 mL / 1000 uL) * (1 ppm / 1 ug/mL) = 1.97 ppm
So, the quantity of gluten in the remaining solution is approximately 1.97 ppm after taking out 2uL.