chemistry lab

An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the experiment? What is the final pH?

If needed: TRIS(Trizma base) mw= 121.1; pka= 8.3; TRIS-HCl mw= 157.6; pka= 8.3

I kind of have an idea. I start out with the hendersn hasselbalch equation and solve for the ratio ([Tris] / [Tris HCl]). I need help doing this first before i can proceed with the rest of the problem.

Thank you very much.

  1. 0
  2. 0
  3. 12
asked by alan

Respond to this Question

First Name

Your Response

Similar Questions

  1. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of

    asked by alan on February 5, 2008
  2. chemistry

    . An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso

    asked by pankaj on July 9, 2015
  3. Biochemistry

    An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso

    asked by Christine on June 26, 2016
  4. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of

    asked by alan on February 2, 2008
  5. chemistry

    This is my third time to ask this and no one has answered. Please help. An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result

    asked by alan on February 3, 2008
  6. Biochemistry

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme esterase catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base, which is the

    asked by Melissa on February 7, 2011
  7. chemistry Please Help Dr.BOB

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme (i.e., esterase)-catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction? (For Tris base,

    asked by Kim on February 8, 2011
  8. Biochemistry

    You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1. What is the concentration of the components (Tris and Tris-H+) in the final buffer solution?

    asked by Alberto on July 3, 2017
  9. Molecular Bio

    You are planning to digest human genomic DNA with the EcoRI restriction enzyme but realize you do not have any 10X buffer available to add to the reaction. The components of the 10X buffer are: 50 mM NaCl, 100 mM Tris, 10 mM

    asked by D. on February 13, 2014
  10. chemistry

    what is the pH of the buffer when 4ml of 1M HCl is added to a 100ml buffer solution made using 5ml 1M Na2HPO4 and 5ml 1M NaH2PO4? The pKa for H+ + HPO4- ==> H2PO4 is 6.82. What would happen to the pH if the 100ml buffer had been

    asked by matt on August 23, 2010

More Similar Questions