# biochemistry lab

A) If you make up a solution of 50 mL of 0.1 M TRIS in the acid form, what will be the pH?

B) If you add 2 mL of 1M NaOH to the solution in part A, what will be the pH?

If it is needed, here is some addition information: TRIS pka= 8.3
mw of TRIS-HCL= 157.6 g/mol;
mw TRIS (Trizma base)= 121.1 g/mol

Thank you!

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