You have a broth that contains 2.7x10^7 cfu/ml/ How many colonies would you expect to count on a plate if you spread our:

a. 1.0ml?
b. 0.1ml?

To determine the number of colonies on a plate when spreading a specific volume of broth, you need to know the concentration of the broth and apply the dilution factor. In this case, the concentration is given as 2.7x10^7 cfu/ml, where "cfu" stands for colony-forming units.

a. To calculate the expected number of colonies when spreading 1.0ml of broth:

- Multiply the concentration by the volume: 2.7x10^7 cfu/ml * 1.0 ml = 2.7x10^7 cfu
- Therefore, you would expect to count 2.7x10^7 colonies on the plate.

b. To calculate the expected number of colonies when spreading 0.1ml of broth:

- Multiply the concentration by the volume: 2.7x10^7 cfu/ml * 0.1 ml = 2.7x10^6 cfu
- Therefore, you would expect to count 2.7x10^6 colonies on the plate.

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