If I had an experiment where I was testing how the membrane permeability is affected by ethanol, and by doing this had to observe thin onion slides with different ethanol concentrations and had to count how many nuclei were seen through the microscope once being stained. My results showed that at higher concentrations of ethanol, more nuclei were observed. How would I make a conclusion from the data I collected. I don't really understand why increasing the ethanol concentration means more nuclei is seen in the cells.
talk about the ethanol dissolving the phospholipid bi layer of the membrane, and the protein as the tertiary structure is ruptured. Also reliability- didn't do any repeats etc, you can use repeats to make an average so it is more accurate. do your results support the hypothesis-ive also got it tommorow and this is what you need to know
To make a conclusion from your data, you can analyze the relationship between ethanol concentration and the number of nuclei observed. Based on your results showing that at higher concentrations of ethanol, more nuclei were observed, you can make the following conclusion:
Increasing the ethanol concentration leads to an increase in the number of nuclei observed in the cells.
Possible explanations for this observation could be:
1. Disruption of cell membrane: Ethanol is known to disrupt the lipid bilayer of cell membranes. At higher concentrations, it is possible that ethanol is increasing the permeability of the onion cell membranes, allowing the nuclear stain to enter the cells and making the nuclei more visible under the microscope.
2. Cell death and lysis: Ethanol is also known to have a toxic effect on cells. At higher concentrations, ethanol may cause cell death and subsequent lysis, leading to the release of cellular contents, including the nuclei. This would result in an increased number of visible nuclei.
It is important to note that further investigation and experiments would be needed to confirm these explanations and fully understand the underlying mechanisms. Additionally, it is crucial to consider potential limitations of the experiment and ensure that controlling variables were adequately addressed.
To make a conclusion from your data, you can start by analyzing the observed trend: increasing ethanol concentration results in more nuclei being seen in the cells. From this observation, you can make the following conclusion:
Conclusion: The increased number of nuclei seen in cells at higher concentrations of ethanol suggests that ethanol affects membrane permeability in such a way that it leads to an increase in the visibility of cell nuclei.
To understand why increasing the ethanol concentration results in more nuclei being observed, it is important to consider the properties of ethanol and how it affects cellular structures. Ethanol is known to disrupt cell membranes and increase their permeability. It can dissolve lipids, which are the main components of cell membranes, making them more fluid to some extent.
When you stained the thin onion slides with different ethanol concentrations and observed them under a microscope, the increased permeability caused by ethanol may have allowed the staining dye to penetrate the cells more easily, leading to the visibility of more nuclei. Ethanol might have facilitated the dye to enter the cells by breaking down or loosening the membrane structure, allowing the stain to reach the nuclei.
It is important to note that this conclusion is based on the observed data and the known effects of ethanol on cell membranes. However, to ensure the accuracy and reliability of your conclusion, it is crucial to consider other factors that could potentially influence the results, such as the staining technique, the quality of the slides, and any other experimental variables. Additionally, it would be beneficial to conduct further experiments and gather more data to support and strengthen your conclusion.