Prepare a 0.025M solution of Glucose by diluting a 1M Glucose solution. What and how much did you mix together?
Use the dilution formula.
mL1 x M1 = mL2 x M2
To prepare a 0.025M solution of Glucose by diluting a 1M Glucose solution, you will need to calculate the volume of the 1M solution and the volume of solvent (usually water) required.
Here are the steps:
Step 1: Determine the volume of the 1M Glucose solution needed.
To do this, you need to calculate the dilution factor. The dilution factor is equal to the final concentration divided by the initial concentration:
Dilution factor = Final concentration / Initial concentration
In this case, the dilution factor is:
Dilution factor = 0.025M / 1M = 0.025
Now, you need to divide the final volume (Vf) with the dilution factor:
V1 * 0.025 = Vf
You can rearrange the equation to solve for V1 (the volume of the 1M Glucose solution needed):
V1 = Vf / 0.025
Step 2: Determine the volume of solvent (water) needed.
You need to subtract the V1 (volume of the 1M solution) from the Vf (final volume) to find the volume of solvent required:
Volume of solvent = Vf - V1
Step 3: Mix the Glucose solution and the solvent together.
Add the calculated volume of the 1M Glucose solution (V1) to the calculated volume of solvent. Mix them together thoroughly.
So, to prepare a 0.025M solution of Glucose, you need to mix the calculated volume of the 1M Glucose solution (V1) with the calculated volume of solvent.
To prepare a 0.025M solution of Glucose, you will need to dilute the 1M Glucose solution. Dilution involves mixing a measured volume of the concentrated solution (in this case, 1M Glucose solution) with a certain volume of a solvent (in this case, water) to obtain the desired concentration.
To calculate the volumes required for dilution, you can use the formula:
C1V1 = C2V2
Where:
C1 = Initial concentration of the solution (1M)
V1 = Initial volume of the solution (unknown)
C2 = Final concentration of the solution (0.025M)
V2 = Final volume of the solution (unknown)
For this example, we want to dilute the 1M Glucose solution to obtain a 0.025M solution. Let's assume the final volume of the diluted solution is 1 liter (1000 ml).
Plug the known values into the dilution formula:
(1M) x (V1) = (0.025M) x (1000 ml)
Rearrange the equation to solve for the initial volume (V1):
V1 = (0.025M) x (1000 ml) / (1M)
V1 = 25 ml
This means you need to take 25 ml of the 1M Glucose solution and mix it with enough water to reach a final volume of 1 liter (1000 ml).