Chemistry-help

Hi,
I have a question on Gas Chromatography(GC).
The following were injected into the GC system: -

1) 1% sample solution in acetone
2) a 500 times dilution of item 1)

The retention time of the main peak in the diluted sample solution 2) shifted by ~ 2min.
The expected area count of the main peak is also half of what is theoretically calculated. (i.e. I would expect the area count of the main peak in the diluted solution to be 500 times less than the sample solution.)

Can you suggest possible reasons for this difference in retention time between the sample and the diluted sample?
Does this shift in retention time happen to some samples that are too diluted?

Thank you.

  1. 👍 0
  2. 👎 0
  3. 👁 156

Respond to this Question

First Name

Your Response

Similar Questions

  1. Science

    Hormones and Homeostasis 1) The human endocrine system is made up of which of the following structures? :Vessels. :Receptors. :Glands.*** :Chambers. 2. Which of the following best describes the function of the endocrine system?

  2. Science The Human Body PLEASE HELP!!!!!!!!!!!

    the human endocrine system is made up of which of the following structures? (1 point) vessels receptors* glands chambers 2. Which of the following best describes the function of the endocrine system? (1 point) It secretes chemical

  3. chemistry

    Consider a system consisting of a cylinder with a movable piston containing 10^6 gas molecules at 298K at a volume of 1L. Consider the following descriptions of this system: 1. Initial system 2. Starting from the initial system,

  4. chem

    What would be the effect of raising the temperature and increasing the carrier gas flow rate on the ability to resolve two closely spaced peaks in gas chromatography? -I was thinking that the width or the maximum peak of each

  1. (2-8) Chemistry - Science (Dr. Bob222)

    Which of the following statements is correct? A) The internal energy of a system increases when more work is done by the system than the heat that flows into the system. B) The system does work on the surroundings when an ideal

  2. Chemistry

    10.0 mol of ammonia gas is injected into a 4.0 L container. At equilibrium 1.2 mol of nitrogen gas is found in the container. The number of moles of ammonia gas left in the container must be which of the following? N2(g) + 3H2(g)

  3. chemistry

    Question: Consider this molecular-level representation of a gas. There are: 5 orange gas particles 6 blue gas particles 3 green molecules of gas (two particles attached together) If the partial pressure of the diatomic gas is

  4. Chemistry

    A 108 mL gas sample has a mass of 77.96 mg at a pressure of 1140 mmHg and a temperature of 183 °C. Assume ideal gas behavior and a completely insulated system, and please don’t forget units with your answers. The system is

  1. language

    1.which factor is most helpful in helping technology to progress? a) unintended consequences b) a better understanding of the natural world c) risk-benefit analysis d)obsolete technologies 2. an oven is a technological system.

  2. Chemistry

    Why is a Gas Chromatography separation more efficient than a fractional distillation? What characteristics must the liquid stationary phase have? describe a method for identifying a compound using GC analysis. If you could answer

  3. Mobility and Rf

    We did a column chromatography then a TLC on extracted spinach pigments. Question: Why are the chlorophylls less mobile on column chromatography and why do they have lower Rf values than the carotenes? My answer: The chlorophylls

  4. Science!

    An oven is a technological system. Which of the following describes an input into this system? A. Setting the temperature B. Burning gas releases heat. C. A thermostat monitors the temperature and increases the gas flow when the

You can view more similar questions or ask a new question.