# Biochemistry

Describe the full preparation of 1.0L 0.2mol/L pH 4.4 acetate buffer. (pka=4.76, Mr Na acetate 82, glacial acetic acid is 17.4mol/L)

1. 👍 0
2. 👎 0
3. 👁 338
1. For 1 L of 0.2 M buffer, you want
acid + base = 0.2
pH = pKa + log(base)/(acid)
4.40 = 4.76 + log(base/acid)
base/acid = 0.436 but you should confirm that. I estimated here and there.
That gives you two equations; solve them simultaneously for acid and base concnc.
eqn 1 is base + acid = 0.2 mol
eqn 2 is base/acid = 0.436 mol

Solve for (base) = ??
Solve for (acid) = ??
Then M acid = moles/L. You know moles and M, solve for L of the 17.4 stuff you want.
For the base, I would solve for grams = moles x molar mass.
I assume you can handle the details.

1. 👍 0
2. 👎 0

## Similar Questions

1. ### Chemistry (buffers)

Say, for example, that you had prepared a Buffer C, in which you mixed 8.203 g of sodium acetate, NaC2H3O2, with 100.0 mL of 1.0 M acetic acid. a. What would be the initial pH of Buffer C? HOW???????

2. ### BIOCHEMISTRY

Calculate the formal concentrations of acetic acid and sodium acetate necessary to prepare a buffer solution of pH 5 that is 0.20M in total acetate?

3. ### chem

Which of the following could be added to a solution of sodium acetate to produce a buffer? ? A) acetic acid only B) acetic acid or hydrochloric acid C) potassium acetate only D) sodium chloride or potassium acetate E) hydrochloric

4. ### Quantitive Analysis

You are asked to prepare 500. mL of a 0.250 M acetate buffer at pH 4.90 using only pure acetic acid (MW=60.05 g/mol, pKa=4.76), 3.00 M NaOH, and water. Answer the following questions regarding the preparation of the buffer.

1. ### school

What is the pH of 0.1 M formic acid solution? Ka=1.7„e10-4? What is the pH value of buffer prepared by adding 60 ml of 0.1 M CH3COOH to 40 ml of a solution of 0.1 M CH3COONa? What is the pH value of an acetate buffer (pK=4.76)

2. ### Chemistry

Describe how to prepare 10 mL of 0.200 acetate buffer, pH 5.00, starting with pure acetic acid and solutions containg 3 M HCl and 3 M NaOH. Include both quantitate and qualitative details. I know that a strong base reacts

3. ### Biochemistry

Show Calculations used in mkaing 250mL of 0.5M sodium acetate ph 4.7. You are given: 1M acetic acid (weak acid) 1M sodium hydroxide (strong base) Sodium acetate buffer: ph 4.7 acetic acid pKa: 4.7 So far this is what I've gotten:

4. ### biochemistry

perform the calculation required for100ml of 0.2 M sodium acetate buffer of pH 5.0. If you are given a bottle of sodium acetate acetate(mw =82 and a bottle of concentrated HCl of normality =11.7). After the calculation, prepare

1. ### chemistry

Calculate the volume of 1.00 M acetic acid solution and mass of sodium acetate needed to make 250 mL of a 0.450 M acetic acid/acetate buffer solution with a pH of 4.00. (The pKa is 4.74 for acetic acid).

2. ### Chemistry - Buffers

A buffer is formed by adding 500mL of .20 M HC2H3O2 to 500 mL of .10 M NaC2H3O2. What would be the maximum amount of HCl that could be added to this solution without exceeding the capacity of the buffer? A. .01 mol B. .05 mol C.

3. ### chemistry 11

Food manufacturers sometimes add calcium acetate to puddings and sweet sauces as a thickening agent. What volume of 0.500 mol/L calcium acetate, Ca(CH3COO)2 (aq), contains 0.300 mol of acetate ions? So I've been over this a

4. ### Chemistry

Calculate pH of an acetate buffer containing 27.5 mM acetic acid (pKa is 4.74) and 50 mM sodium acetate. When a 30 mM aq solution of sodium acetate was prepared in the lab it had a pH of 8.8. Calculate the amount of acetic acid