Solvent

Question: A sample was placed on a chromatography column. Methylene chloride was used as the eluting solvent. All of the components eluted off the column but no separation was observed. What must have been happening during this experiment:? How would you change the experiment to overcome this problem?

My answer: The components must all have been fairly nonpolar and the methylene chloride, a solvent of intermediate polarity, eluted them all at the same time. A nonpolar solvent should be used to overcome this problem.

Thanks for checking this.

Sheryl


Looks like I already submitted this so never mind.

Sheryl

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