You need to prepare medium for your culture cells. Your salt solution is 10x concentration, dilute to 1x for use. You also need to add fetal bovine serum for a final concentration of 10%. What would you add of each for the correct final concentrations in a liter of media?
To prepare the medium for your culture cells, you need to dilute your 10x salt solution to 1x concentration and add fetal bovine serum (FBS) to achieve a final concentration of 10%.
To calculate the amounts needed, you first need to determine the volume of the final medium, which in this case is 1 liter (1000 mL).
Let's start with the 10x salt solution. Since you want to dilute it to 1x concentration, you need to add 1/10th of the volume of the 10x solution to achieve the desired concentration.
So, for a final volume of 1 liter, you'll need to add 1/10 * 1000 mL = 100 mL of the 10x salt solution.
Next, let's calculate the volume of fetal bovine serum (FBS) required to achieve a final concentration of 10%.
A 10% concentration means that 10% of the final volume should be FBS. To calculate the volume of FBS for a 10% concentration, you multiply the final volume by 10% (0.10).
Therefore, for a final volume of 1 liter, you'll need to add 1 liter * 0.10 = 100 mL of fetal bovine serum.
In summary, to prepare a liter of medium with a 1x salt concentration and a final concentration of 10% fetal bovine serum, you should add 100 mL of the 10x salt solution and 100 mL of fetal bovine serum to 800 mL of water or additional basal medium.