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biochemistry

How do you calculate the PH of a buffer solution prepared by mixing 75 mL of 1.0 M lactic acid and 25 mL of 1.0 M sodium lactate?

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  1. (acid) = 1M x 75/100 = ??
    (salt) = 1 M x 25/100 = ??
    Plug into the Henderson-Hasselbalch equation.

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  2. 4.34

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  3. the pKa of lactic acid is 3.86

    pH=3.86+ log[(25x1.0)÷(75x1.0-3)]

    pH = 3.38

    the pKa of sodium lactate 3.78

    pH=3.78+ log[(25x1.0)÷(75x1.0-3)]

    pH = 3.30

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