I have a 2.5g sample in 12.5ml solution.I'm supposed to do a spike into this solution with the sample.If my standard concentration is 2000ppm, how much of this solution should I use such that I get 200ppm spike in sample?
To calculate the volume of the standard solution required to create a 200 ppm spike in your sample, you will need to know the volume of your sample and the concentration of the standard solution. Here's how you can calculate it:
1. Calculate the amount of spike required in the sample:
- The desired spike concentration is 200 ppm (parts per million).
- Multiply the sample volume (12.5 ml) by the desired spike concentration (200 ppm) to get the amount of spike required: 12.5 ml x 200 ppm = 2500 ppm·ml.
2. Calculate the volume of the standard solution required:
- The concentration of the standard solution is given as 2000 ppm (parts per million).
- Divide the amount of spike required (2500 ppm·ml) by the concentration of the standard solution (2000 ppm) to get the volume required: 2500 ppm·ml / 2000 ppm = 1.25 ml.
So, you will need to use 1.25 ml of the standard solution to create a 200 ppm spike in your sample.