TALKING ABOUT EXTRACTING DNA
eXPERIMENT: MASH FRUIT UP WITH COLD ISOPROPYL ALCOHOL, SALT, WATER, AND DETERGENT THEN EXTRACT DNA USING A WOODEN SPLINT.
WHEN EXTRACTING DNA FROM STRAWBERRIES WHY CAN WE NOT SEE IT IN MASHED UP STRAWBERRIES BUT YOU CAN SEE IT ON A WOODEN SPLINT? WHY DID ALCOHOL NEED TO BE COLD AND WHY DID WE HAVE TO POUR ALCOHOL SLOWLY DOWN INTO MASHED STRAWBERRIES?
i'M NOT SURE HOW TO ANSWER THESE QUESTIONS....HELP!!!
When extracting DNA from strawberries, you may not be able to see the DNA in the mashed-up strawberries because the DNA is hidden within the cell structure of the fruit. The cells have a protective layer called the cell membrane, which holds the DNA inside and prevents it from being easily visible.
To extract the DNA from the strawberries, the experiment uses a mixture of cold isopropyl alcohol, salt, water, and detergent. The purpose of using cold alcohol is to help separate the DNA from other components of the cell. When the cold alcohol is added to the mashed strawberries, it creates a layer on top of the mixture.
The reason we pour the alcohol slowly down into the mashed strawberries is to create a gradient. As the alcohol flows down, it mixes with the strawberry mixture and causes the DNA to precipitate out of the mixture. By pouring the alcohol slowly, we allow the DNA to slowly rise through the mixture and form a visible layer on top of the alcohol. This makes it easier to see and collect the DNA.
The wooden splint is used to collect the extracted DNA. When you dip the wooden splint into the mixture and then lift it out slowly, the DNA molecules stick to the wooden surface. This is because DNA molecules are negatively charged and are attracted to the wooden surface, which is slightly positive.
In summary, the cold alcohol is used to separate the DNA from the other components, and pouring it slowly helps the DNA to precipitate out of the mixture. The wooden splint is used to collect the DNA molecules, which stick to its surface.