You perform gel filtration and measure an elution volume of 25 ml. When you calibrated the same gel filtration column, the elution volumes of proteins of known molecular weights were as follows: 400,000 Da, 10 ml; 250,000 Da, 20 ml; 150, 000 Da, 30 ml; 100,000 Da, 40 ml. Plot the logarithm of the molecular weights of the known protein versus elution volume

Question

You perform gel filtration and measure an elution volume of 25 ml. When you calibrated the same gel filtration column, the elution volumes of proteins of known molecular weights were as follows: 400,000 Da, 10 ml; 250,000 Da, 20 ml; 150, 000 Da, 30 ml; 100,000 Da, 40 ml. Plot the logarithm of the molecular weights of the known protein versus elution volume

Answer 1

It is impossible to draw graphs on this forum.

Answer 2

To plot the logarithm of the molecular weights of the known proteins versus elution volume, we need to calculate the logarithm of the molecular weights and then plot them against the corresponding elution volumes.

Let's start by calculating the logarithm of the molecular weights:

Molecular weight (Da) Logarithm
--------------------- ----------
400,000 log(400,000)
250,000 log(250,000)
150,000 log(150,000)
100,000 log(100,000)

Using a calculator or software that can calculate logarithms, we can determine the logarithm values for each molecular weight.

Now we can plot the data points on a graph, with the elution volume along the x-axis and the logarithm of the molecular weight along the y-axis. The x-axis represents the elution volume, which we have already calculated as 10 ml, 20 ml, 30 ml, and 40 ml. The y-axis represents the logarithm of the molecular weight.

Once the graph is plotted, we can draw a line connecting the data points to visualize the trend between the logarithm of the molecular weight and elution volume. This line will help to estimate the molecular weight of an unknown protein based on its elution volume in future experiments.