Too make a 4% solution of paraformaldehyde from a 20 % solution.
C1V1=C2V2
(20)(10ml)= (4)(x)
x= 50ml
so I would need to add 45ml of PBS and 4ml of the paraformaldhyde.
Is that right.
You have a 20% solution of paraformaldehyde in what solvent?
Yes, your calculations are correct. To make a 4% solution of paraformaldehyde from a 20% solution, you can use the formula C1V1 = C2V2, where C1 is the initial concentration (20%), V1 is the initial volume (10ml), C2 is the final concentration (4%), and V2 is the final volume (unknown).
By rearranging the formula, you have (20)(10ml) = (4)(x), where x represents the final volume of the 4% solution.
Solving for x, you get:
200ml = 4x
x = 200ml / 4
x = 50ml
So, to make a 50ml solution of 4% paraformaldehyde, you would need to add 45ml of PBS (a buffer solution commonly used in biological experiments) and 5ml of the 20% paraformaldehyde solution.
Your calculations are correct, and you would need to add 45ml of PBS and 5ml of the 20% paraformaldehyde solution to obtain a 4% solution of paraformaldehyde.