ive calculated the amount of change for an enzyme controlled reaction as 1.5625 x 10^-8 mol.min-1 and ive been asked to find the activity of the enzyme in micromoles product formed per min per mg of enzyme how do i do this

i have 10 micro litres of a 5mg ml -1 of the enzyme ???

Any help/explanation would be appreciated !!!

Thank you

To find the activity of the enzyme in micromoles per minute per milligram (μmol/min/mg), you need to follow these steps:

STEP 1: Convert the enzyme concentration from mg/mL to mg/μL:
Since you have 10 μL of enzyme, you can use the given concentration of 5 mg/mL to calculate the amount of enzyme in mg/μL.
5 mg/mL * 10 μL = 50 mg.

STEP 2: Convert the change in moles of product formed to micromoles:
Given the change in moles as 1.5625 x 10^-8 mol.min^-1, you need to convert it to micromoles by multiplying it by 10^6.
1.5625 x 10^-8 mol.min^-1 * 10^6 μmol/mol = 15.625 μmol.min^-1.

STEP 3: Calculate the enzyme activity:
Divide the micromoles of product formed (from Step 2) by the amount of enzyme in milligrams (from Step 1).
15.625 μmol.min^-1 / 50 mg = 0.3125 μmol.min^-1.mg^-1.

So, the activity of the enzyme is 0.3125 μmol.min^-1.mg^-1.