A rock covered with green spots is placed on a screen in a test tube above some bromothymol blue solution; after several hours, the solution remains blue. What can you conclude form this experiment?
AFter doing research, I found out that bromothymol solution is a blue liquid and is an indicator of pH levels and shows if the organism is respiring or not.
So if it's blue, it is not respiring and basic
If the solution is yellow or green, the solution is acidic and the rock is producing CO2.
Why is that in my question at top, the solution stay the same. Why didn't the green spots have any effect?
Based on the given information, if the solution remains blue after several hours, it suggests that the rock covered with green spots did not have any effect on the bromothymol blue solution. This could mean that the green spots on the rock did not produce enough carbon dioxide (CO2) to cause a change in the pH of the solution.
It is possible that the green spots on the rock are not actively respiring or producing enough CO2 to lower the pH of the surrounding solution. Alternatively, the green spots may not contain any substances that react with bromothymol blue or affect its color change.
It is important to note that without further information or experimentation, it is difficult to determine the exact reason why the green spots did not have an effect on the bromothymol blue solution in this specific experiment.
Based on the information provided, there might be a couple of possibilities for why the green spots on the rock did not have any effect on the bromothymol blue solution during the experiment.
1. Inactive or non-respiring organisms: It is possible that the green spots on the rock were caused by non-respiring or inactive organisms. Bromothymol blue solution changes color in response to the presence of carbon dioxide (CO2), which is produced during respiration. If the organisms on the rock were not actively respiring or producing CO2, they would not have caused a change in the color of the solution.
2. Neutral pH levels: Another possibility is that the green spots on the rock did not significantly affect the pH level of the bromothymol blue solution. Green spots might not have released enough CO2 to cause a noticeable change in the pH level of the solution. If the pH level remained neutral (neither acidic nor basic), the bromothymol blue solution would have remained blue.
It is important to note that without further information or additional experimentation, it is difficult to definitively conclude why the green spots on the rock did not affect the bromothymol blue solution.