How would you prepare 100 ml of 0.9% w/v saline?
To prepare 100 ml of 0.9% w/v (weight/volume) saline solution, you will need the following materials and ingredients:
1. Sodium chloride (NaCl)
2. Distilled water
3. A 100 ml volumetric flask or measuring cylinder
4. Weighing scale or balance
Now, let's break down the steps to prepare the saline solution:
Step 1: Calculate the amount of sodium chloride needed.
To determine the amount of sodium chloride required, you need to know the desired final concentration, which is 0.9% w/v. This means that for every 100 ml of solution, 0.9 grams of sodium chloride should be dissolved.
To calculate the amount of sodium chloride needed:
(0.9 g / 100 ml) × 100 ml = 0.9 g
Step 2: Weigh the required amount of sodium chloride.
Using a weighing scale or balance, measure 0.9 grams of sodium chloride accurately.
Step 3: Add the sodium chloride to the 100 ml volumetric flask.
Carefully transfer the measured 0.9 grams of sodium chloride into the 100 ml volumetric flask.
Step 4: Add distilled water to make a total volume of 100 ml.
Fill the volumetric flask with distilled water up to the 100 ml mark. Make sure to add the water slowly and avoid introducing any air bubbles.
Step 5: Mix the solution thoroughly.
Securely cap the flask and mix the solution thoroughly by gentle shaking or swirling until the sodium chloride is completely dissolved.
Now, you have successfully prepared 100 ml of 0.9% w/v saline solution.