How much Tris-Acetate-EDTA (TAE) stock buffer (3.25 M) is required to make 400 ml of solution that has a concentrations of 250 mM? How much water?
ml1 x M1 = mL1 x M2
mL1 x 3.25M = 400 mL x 0.250
Solve for mL1 volume, take that volume of the stock, transfer to a 400 mL volumetric flask and make to the mark with distilled water. Stopper. mix thoroughly, label. Done.
To determine how much Tris-Acetate-EDTA (TAE) stock buffer is required to make 400 ml of a solution with a concentration of 250 mM, we can use the following formula:
(C1)(V1) = (C2)(V2)
Where:
C1 = Initial concentration of TAE stock buffer (3.25 M)
V1 = Volume of TAE stock buffer to be used
C2 = Final desired concentration (250 mM)
V2 = Final desired volume (400 ml)
Now, let's calculate the volume of TAE stock buffer needed:
(3.25 M)(V1) = (250 mM)(400 ml)
To convert the units from M to mM, we need to multiply by 1000:
(3250 mM)(V1) = (250 mM)(400 ml)
Now, we can solve for V1:
V1 = (250 mM)(400 ml) / (3250 mM)
V1 = (62500 mM*ml) / (3250 mM)
V1 = 19.23 ml (rounded to two decimal places)
Therefore, you would need 19.23 ml of TAE stock buffer to make 400 ml of a solution with a concentration of 250 mM.
To determine how much water is needed, we can subtract the volume of TAE stock buffer from the final desired volume:
Volume of water = Final desired volume - Volume of TAE stock buffer
Volume of water = 400 ml - 19.23 ml
Volume of water = 380.77 ml (rounded to two decimal places)
Therefore, you would need approximately 380.77 ml of water to make the solution.
To calculate the amount of Tris-Acetate-EDTA (TAE) stock buffer required to make a 400 ml solution at a concentration of 250 mM, we can use the following formula:
C1V1 = C2V2
Where:
C1 = initial concentration of the stock buffer (3.25 M)
V1 = volume of stock buffer required
C2 = target concentration of the final solution (250 mM)
V2 = final volume of the solution (400 ml)
First, let's calculate the volume of TAE stock buffer required:
V1 = (C2 * V2) / C1
= (250 mM * 400 ml) / 3.25 M
To convert the concentrations to the same units, we need to convert mM to M by dividing by 1000:
V1 = (0.25 M * 0.400 L) / 3.25 M
≈ 0.0308 L or 30.8 ml
Thus, you would need approximately 30.8 ml of TAE stock buffer to make the desired 400 ml solution at a concentration of 250 mM.
To determine the amount of water needed, subtract the volume of the stock buffer from the final volume of the solution:
Volume of water = V2 - V1
= 400 ml - 30.8 ml
≈ 369.2 ml
Therefore, you would need approximately 369.2 ml of water to make the 400 ml solution with a concentration of 250 mM.