A mixture of amino acids (written as M) was tested against

five known amino acids (written as 1 to 5) and the
chromatogram was made. Which amino acids are found in the
mixture M?

How do you expect anyone to answer this? You have no data, in fact, you have no information at all other than that it is an amino acid mixture. We aren't clairvoyant.

To determine which amino acids are found in the mixture M, you can compare the chromatogram of the mixture with the chromatograms of the known amino acids (1 to 5).

Here's how you can do it:

1. Obtain the chromatogram of the mixture M. This chromatogram shows the separation and distribution of the different amino acids in the mixture.

2. Obtain the chromatograms of the known amino acids (1 to 5). These chromatograms serve as references to identify the specific amino acids present in the mixture M.

3. Compare the chromatograms visually. Look for peaks or patterns that match between the mixture M and the known amino acids. Peaks indicate the presence of specific amino acids.

4. Identify the amino acids present in the mixture M by matching the peaks or patterns with those in the known chromatograms. Each amino acid has a characteristic retention time, which can help in their identification.

5. Once you have identified the amino acids present in the mixture M, you can assign a label or name to each amino acid based on the matching peaks or patterns.

By following these steps, you can determine which amino acids are found in the mixture M by comparing its chromatogram with the reference chromatograms of the known amino acids.