A protein solution (0.3ml) is diluted with 0.9ml water. 0.5ml is taken and reacted with 4.5ml of reagent and the resulting colour allowed to develop. The Abs at 540nm was 0.18 in a 1cm cuvette. A standard protein solution (0.5ml containing 2mg) gave an absorbance reading of 0.12 when reacted in the same way. How much protein would you have in 100ml of the undiluted original sample?
Absorbance of 2 mg std = 0.12
Absorbance of unk = 0.18 so
2 mg x (0.18/0.12) = 3 mg protein in the measured sample. That came from the 1.2 mL diluted sample. The amount in that was
3 mg x (1.2/0.5) = 7.2 mg in the 1.2 mL dilution. In the initial 0.3 mL sample there is 7.2 x (0.3/1.2) = 1.8. In 100 mL there will be
1.8 x (100/0.3) = ?
I don't know
Well, this sounds like a serious question. Let me put on my serious clown glasses and give you a serious answer, with a touch of humor.
To calculate the amount of protein in 100ml of the undiluted original sample, we need to do a little protein math. Don't worry, no protein equation will make you break out in hives!
First, let's calculate the concentration of the standard protein solution (0.5ml). We know it contains 2mg of protein and gives an absorbance reading of 0.12. Now, apply a sprinkle of math magic and calculate the concentration:
Concentration = amount of protein / volume of solution
Concentration = 2mg / 0.5ml
Concentration = 4mg/ml
Now, let's determine the dilution factor of the protein solution. We know that 0.3ml of the protein solution was diluted with 0.9ml of water, which gives us a dilution factor of 1:4. Why was the water always waiting for someone to dilute things? Because it loved to make things less "concentrated"!
Next, we'll determine the concentration of the protein solution after dilution. Multiply the concentration of the standard protein solution (4mg/ml) by the dilution factor (4):
Concentration after dilution = Concentration x Dilution factor
Concentration after dilution = 4mg/ml x 4
Concentration after dilution = 16mg/ml
Now comes the grand finale! To find the amount of protein in 100ml of the undiluted original sample, we multiply the concentration after dilution by the volume:
Amount of protein = Concentration after dilution x Volume
Amount of protein = 16mg/ml x 100ml
Drumroll, please! The amount of protein in 100ml of the undiluted original sample is...
1600mg! Ta-da! Now, that's a clownishly serious amount of protein! I hope this answer brings a smile to your face along with a dash of protein knowledge.
To calculate the amount of protein in 100ml of the undiluted original sample, we can follow these steps:
Step 1: Calculate the dilution factor for the protein solution.
The dilution factor is given by dividing the total volume of the diluted solution by the volume of the original solution.
Dilution factor = (Volume of diluted solution) / (Volume of original solution)
Dilution factor = (0.3ml + 0.9ml) / 0.3ml
Dilution factor = 4
Step 2: Calculate the concentration of the protein in the diluted solution.
The concentration of the protein in the diluted solution can be determined using the Beer-Lambert law, which states that absorbance is directly proportional to concentration.
Concentration (diluted) = Absorbance (diluted) / Absorbance (standard) * Concentration (standard)
Concentration (diluted) = 0.18 / 0.12 * 2mg
Concentration (diluted) = 3mg/ml
Step 3: Calculate the concentration of the protein in the undiluted original sample.
Since the dilution factor in Step 1 was 4, the concentration of the protein in the undiluted original sample will be four times the concentration of the diluted solution.
Concentration (undiluted) = Concentration (diluted) * Dilution factor
Concentration (undiluted) = 3mg/ml * 4
Concentration (undiluted) = 12mg/ml
Step 4: Calculate the amount of protein in 100ml of the undiluted original sample.
Amount of protein = Concentration (undiluted) * Volume (undiluted)
Amount of protein = 12mg/ml * 100ml
Amount of protein = 1200mg
Therefore, there would be 1200mg of protein in 100ml of the undiluted original sample.
To calculate the amount of protein in 100ml of the undiluted original sample, we need to follow these steps:
Step 1: Calculate the dilution of the protein solution.
The original protein solution was diluted with 0.9ml of water after adding 0.3ml. So, the total volume of the diluted solution is 0.3ml + 0.9ml = 1.2ml.
Step 2: Calculate the concentration of the protein solution.
To find the concentration, we divide the amount of protein (in mg) by the volume of the solution (in ml).
Since the standard protein solution (0.5ml) with 2mg of protein gave an absorbance reading of 0.12, we can determine its concentration as follows:
Concentration (mg/ml) = Amount of protein (mg) / Volume of solution (ml)
Concentration = 2mg / 0.5ml = 4mg/ml
Step 3: Calculate the protein concentration in the diluted sample.
Since the diluted protein solution has a total volume of 1.2ml, we can calculate the protein concentration as follows:
Protein concentration (mg/ml) = Concentration of standard protein solution (mg/ml) * Dilution factor
The dilution factor is the ratio of the final volume to the initial volume of the protein solution.
Dilution factor = Total volume of diluted solution (ml) / Initial volume of protein solution (ml)
Dilution factor = 1.2ml / 0.3ml = 4
Protein concentration = 4mg/ml * 4 = 16mg/ml
Step 4: Calculate the protein content in 100ml of the undiluted original sample.
To find the protein content in 100ml, we multiply the protein concentration (mg/ml) by the volume of the sample (ml).
Protein content = Protein concentration (mg/ml) * Volume of sample (ml)
Protein content = 16mg/ml * 100ml = 1600mg
Therefore, there would be 1600mg of protein in 100ml of the undiluted original sample.