Grade 11 learners want to investigate which enzyme is responsible for chemical digestion of starch into glucose in humans. They are provided with two different enzymes (carbohydrase and Protease)starch powder,warm water at about 37°C and basic glass apparatus and chemical to test for glucose
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Independent variable
To investigate which enzyme is responsible for the chemical digestion of starch into glucose in humans, the grade 11 learners can follow the steps below using the provided materials:
1. Start by preparing two test tubes, labeled A and B.
2. In test tube A, mix a small amount of the starch powder with warm water at approximately 37°C. This will create a starch solution.
3. In test tube B, add the same amount of starch powder but do not add any enzymes. This will serve as a control, allowing comparison between the effects of different enzymes.
4. Add the carbohydrase enzyme to test tube A. Carbohydrase is the enzyme responsible for breaking down carbohydrates like starch into simpler sugars, including glucose.
5. Leave both test tubes at 37°C for a certain period of time, depending on the instructions provided for the carbohydrase enzyme. This is to allow sufficient time for the enzyme to digest the starch.
6. After the digestion period is complete, add a few drops of the chemical solution provided to test tubes A and B. This chemical is used to test for the presence of glucose.
7. Observe the color change in each test tube. If glucose is present, the chemical will change color indicating its presence.
8. If test tube A, which contains the carbohydrase enzyme, shows a color change indicating the presence of glucose, it suggests that the carbohydrase enzyme is responsible for the chemical digestion of starch into glucose.
9. If test tube B, the control without any enzyme, does not show a color change indicating glucose, it confirms that the presence of glucose in test tube A is a result of the carbohydrase enzyme's activity.
By carrying out this investigation, the grade 11 learners will be able to determine which enzyme, carbohydrase in this case, is responsible for the chemical digestion of starch into glucose in humans.