Read the scenario.

A graduate student is running a PCR reaction to study DNA extracted from an Egyptian mummy. The reaction does not seem to be working, so he checks his notes to see what he added to the reaction mix. He find the following list: mummy DNA, DNA polymerase, and DNA nucleotides.

What is the error in the setup?

He forgot to add DNA ligase.

He should have added DNA ligase instead of DNA polymerase.

He forgot to add primers.

He should have recognized that PCR cannot work on DNA extracted from a mummy.

I think the answer is A

  1. 👍 0
  2. 👎 0
  3. 👁 923
  1. You can only pick one?

    He forgot to add primers would be my choice, DNA primers and not RNA primers with the latter being used in normal DNA replication in cells. There are no Okazaki fragments that need to be annealed, so no need for DNA ligase. Not being able to perform PCR on mummy DNA maybe an option, but I don't think it is the right answer choice.

    1. 👍 2
    2. 👎 0
  2. This guy is right it is He forgot to add primers. I just took the test got it right.

    1. 👍 2
    2. 👎 0
  3. Same, it's the right answer.

    1. 👍 1
    2. 👎 0

Respond to this Question

First Name

Your Response

Similar Questions

  1. science

    Read the scenario and answer the following questions: a. How could the scenario have been prevented? b. How should the student respond in the scenario to minimize the harm? During an experiment, Carin carefully pours solution A

  2. MICRO

    The size of the amplified DNA fragment generated during PCR is determined by: many cycles are performed. b. the size of the template c. the location to which the primers anneal. d. how much Taq is used. I believe the answer

  3. Maths/statistics

    the systolic blood pressure of MAAUN under graduate.student is distributed normally with arithmetic mean SD 140mmhg and 20mmHg respectively.if a student was selected at random, (A).what is the probability his/her SBP is more than

  4. Math

    Out of 40 student in a class 16 study English ,22study account & 26 eco. 5 study English & eco ,14 Account & eco 2 study all the three subject if each student studies at least one of the three subject. Find the number of student

  1. genetics

    Which of the following does not occur during the PCR reactions? A.Changes in the reaction temperature by a thermocycler B.Denaturation at high temperatures C.Synthesis of oligonucleotide primers D.Extension of primers by DNA

  2. Chem

    Chalcocite is an ore from which copper can be extracted for commercial use. The formula for this important ore is Cu2S. In the laboratory it is formed by the following reaction: 2Cu + S → Cu2S The reaction takes place in

  3. English

    Why do you study English? 1. I study English to chat with foreigners. 2. I study English to get 100 points on the English test in the final exam. 3. I study English to read technical English books. 4.I study English to enter

  4. Scientists PCR

    Scientists use polymerase chain reaction (PCR) to amplify a segment of DNA into many copies. to insert new nucleotide segments into a plasmid. to make a complementary strand of DNA. to link together multiple fragments of DNA WHICH

  1. Science

    A scientist goes to a large international conference and hears about a new discovery. The scientist is very excited about these new findings and decides to verify the other scientists' results by conducting her own investigations.

  2. Math

    Nigel is participating in a read-a-thon. The number of pages he reads each night follows a geometric sequence. On the second day of the read-a-thon, Nigel read 8 pages. On the fifth day of the read-a-thon, he read 64 pages. Write

  3. Biology

    Which of the following must occur for a mismatch error to be repaired? Which is the best answer, I think it's A, am I right? A)Damaged DNA must be identified by DNA repair proteins B)The sequence of nucelotides in the damaged DNA

  4. statistik and probability

    n a college class of 250 graduating seniors 50 have jobswaiting. 10 are going to medical school, 20 are going to lawschool, and 80 are going to various other kinds of graduateschools. Select one graduate at random. a. What is the

You can view more similar questions or ask a new question.