If we see a DNA sequence in our FASTA file that reads: ATGCCCTTCGGG. What is the corresponding sequence of our double-stranded DNA molecule?
You need to put the numbers corresponding to the ends.
Assuming the the strand is 3 to 5.
DNA is read 3 to 5 and copied 5 to 3
3’ ATGCCCTTCGGG ‘5
5’ TACGGGAAGCCC ‘3
To determine the corresponding sequence of the double-stranded DNA molecule, we need to understand the base pairing rules. In DNA, the four nucleotide bases are adenine (A), thymine (T), cytosine (C), and guanine (G). Adenine pairs with thymine, and cytosine pairs with guanine.
Given the DNA sequence in our FASTA file: ATGCCCTTCGGG, we can identify the complementary sequence by replacing each base with its pairing partner.
The sequence is:
ATGCCCTTCGGG -> TACGGGAAGCCC
Therefore, the corresponding sequence of the double-stranded DNA molecule is TACGGGAAGCCC.