Determine the CFU/ml if 1 ml of the 10-5 dilution is plated and 372 colonies are present following incubation.
Thank you!
cfu/ml = (no. of colonies x dilution factor) / volume of culture plate=372*105 /1ml
To determine the colony forming units per milliliter (CFU/ml), we need to use the dilution factor and the number of colonies observed on the plate.
In this case, the dilution factor is 10^(-5), which means the original sample was diluted by a factor of 10^5 times.
To calculate the CFU/ml, follow these steps:
1. Count the number of colonies on the plate after incubation, which is 372 in this case.
2. Determine the dilution factor. In this case, it is 10^(-5).
3. Divide the number of colonies by the dilution factor to get the CFU/ml. Mathematically, it can be calculated as:
CFU/ml = (Number of colonies) / (Dilution factor)
Applying this to the given values:
CFU/ml = 372 / 10^(-5) = 372 x 10^5 = 372 x 100,000 = 37,200,000 CFU/ml
Therefore, the CFU/ml for the given dilution and colony count is 37,200,000 CFU/ml.