Read the scenario.
Loretta has a sample that contains a mixture of different DNA molecules. She would like to separate them by size to help her determine the length of each molecule.
Which technique should she use?
A- PCR
B- Gel electrophoresis
C- DNA Barcoding
D- Sanger sequencing
D?
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No, the correct answer is B- Gel electrophoresis. Gel electrophoresis is a technique commonly used to separate DNA molecules based on their size. It involves running the DNA sample through a gel matrix using an electric current, causing the DNA fragments to migrate towards the positive pole. The smaller DNA fragments move faster and travel farther, while the larger ones move slower and stay closer to the origin. This separation by size allows for the determination of the length of each DNA molecule.
No, the correct technique that Loretta should use to separate DNA molecules by size and determine the length of each molecule is B- Gel electrophoresis.
Gel electrophoresis is a commonly used technique in molecular biology that separates DNA fragments by their size. It involves placing the DNA sample onto a porous gel and subjecting it to an electric field. The DNA molecules will migrate through the gel according to their size, with smaller molecules traveling faster and farther than larger ones. This separation allows Loretta to determine the size of the DNA molecules present in her sample.
PCR (Polymerase Chain Reaction) is a technique used to amplify a specific segment of DNA, rather than separating different molecules. DNA Barcoding is a method used to identify species by analyzing a specific DNA region, not to separate DNA molecules by size. Sanger sequencing is a technique used to determine the exact nucleotide sequence of a DNA molecule, not to separate DNA molecules by size.