What are two types of materials or observations that would necessitate the use of the stereoscopic microscope?
What organelles are visible with the electron microscope but not with the light microscope?
(we've been studying single celled organisms and organelles).
Well, the optimum range of the iodine used would pose as a serious factor. Or, when studying the peacock feather it is taken into consideration that the purple dye is used in the seperation of the elaxtic band in the back compartment of the brain. I hope this answers the question for you.
To answer the first question, two types of materials or observations that would necessitate the use of a stereoscopic microscope are:
1. Small three-dimensional specimens: The stereoscopic microscope provides a three-dimensional view of the specimen, allowing for observations of its height, width, and depth. This is useful when examining small objects with complex structures, such as insects, minerals, or microelectronic components.
2. Dissection or live specimens: The stereoscopic microscope is optimal for observing specimens that require manipulation or are alive. It allows for a wider working distance, which is the distance between the objective lens and the specimen, enabling the use of tools for dissections or observations of live organisms.
For the second question, the electron microscope can reveal organelles that are not visible using a light microscope. These include:
1. Ribosomes: These small structures, responsible for protein synthesis, are typically not visible with a light microscope due to their size (20-30 nanometers). Electron microscopes, with their higher magnification and resolution, can observe ribosomes.
2. Endoplasmic reticulum (ER): The ER, involved in protein and lipid synthesis, appears as a network of interconnected membranes. While the light microscope can show some parts of the ER, the electron microscope provides a more detailed view of its structure.
3. Golgi apparatus: The Golgi apparatus, involved in modifying, sorting, and packaging proteins, can be seen using electron microscopy due to its intricate membrane system and stacked structure.
4. Mitochondria: Mitochondria, responsible for energy production in cells, are more visible with an electron microscope, revealing their unique double-membrane structure and internal components.
5. Centrioles: These structures play a role in cell division and are not easily visible under a light microscope. However, they become visible with electron microscopy due to their intricate arrangement of microtubules.
Overall, the electron microscope's higher resolution and magnification capabilities enable the visualization of smaller structures within cells that are beyond the limits of light microscopy.