If you had a wheat germ RNA yield of 0.213 g, how much DEPC water would you need to add to make a 20 mg/mL solution of RNA/polysaccharide mixture?

To determine the amount of DEPC water needed to make a 20 mg/mL solution of RNA/polysaccharide mixture, we need to use the given wheat germ RNA yield of 0.213 g. Here's how we can calculate it:

1. Convert the wheat germ RNA yield from grams to milligrams:
- 0.213 g × 1000 mg/g = 213 mg

2. Calculate the volume of DEPC water needed using the formula:
- Volume (V) = Mass (m) / Concentration (C)

The given concentration is 20 mg/mL, and the mass of RNA is 213 mg.
Therefore, V = 213 mg / 20 mg/mL

3. Calculate the volume:
- V = 213 mg / 20 mg/mL
- V = 10.65 mL

Therefore, you would need to add approximately 10.65 mL of DEPC water to make a 20 mg/mL solution of RNA/polysaccharide mixture.