What is the difference between adsorption and partition chromatography?
A. absorption is faster?
B. partitioning is faster
C. absorption can only be used with gaseous analytes
D. partition can only be used with gaseous analytes
E. one typically uses two immiscible liquids with one and not the other
my answer is E. am I correct
No, your answer is not correct. The correct answer is option E.
To understand the difference between adsorption and partition chromatography, we need to have a basic understanding of the two processes:
1. Adsorption chromatography: In this type of chromatography, the separation principle is based on the differences in adsorption affinity between the analyte molecules and the stationary phase (solid). The analyte molecules adsorb onto the surface of the solid stationary phase, while the mobile phase (liquid or gas) carries them through the column. The separation occurs due to the different affinities of the analyte molecules for the stationary phase.
2. Partition chromatography: In partition chromatography, the separation principle is based on the differences in solubility between the analyte molecules and the stationary phase. The stationary phase is typically a liquid-coated solid support or a liquid phase on a solid support. The analyte molecules can partition between the stationary phase and the mobile phase, leading to their separation.
Now, let's go through the given answer options to understand why option E is correct:
A. The speed of adsorption and partition chromatography is not compared in the question, so this statement is irrelevant.
B. The speed of partition chromatography is not compared to adsorption chromatography, so this statement is also irrelevant.
C. Adsorption chromatography can be used with both gaseous and liquid analytes, so this statement is incorrect.
D. Partition chromatography can be used with both gaseous and liquid analytes, so this statement is also incorrect.
E. This statement is correct. In adsorption chromatography, we typically use a solid stationary phase, while in partition chromatography, we use two immiscible liquid phases (one being the stationary phase and the other being the mobile phase).
Therefore, the correct answer is E: One typically uses two immiscible liquids with one (partition chromatography) and not the other (adsorption chromatography).