A mutation of c to t in position 3 and another of G to A in position 7 results in the production of a different sequence of ammino acids work out the new sequence and suggest why it might change the function of the new protein
I get the sequence of
ATAAGCTTT
in Mrna
AUAAGCUUU
wHICH
AUA = lle
AGC = ser
UUU = PHENYLALANINE
The reason I give is that mutant strands lack meth and no protein to syntheis
I look forward to your critique
sorry have you taken the original DNA structure then altered the position 3 & 7 as the mutation, then altered to the mRNA structure, to get the amino acids, it looks like you have done it twice!!!
To determine the new sequence of amino acids, we need to understand the process of transcription and translation. Transcription is the process in which DNA is transcribed into mRNA, while translation is the process in which mRNA is translated into a sequence of amino acids to form a protein.
First, let's take the original DNA sequence and apply the two given mutations:
Original DNA sequence: CCTGCCATCGT
Mutation 1: C to T at position 3
Mutated DNA sequence: CTTGCCATCGT
Mutation 2: G to A at position 7
Final mutated DNA sequence: CTTGCCATACG
Now, we need to transcribe this DNA sequence into mRNA by replacing each nucleotide with its complementary base:
DNA: CTTGCCATACG
mRNA: GAA CGGU AUGC
After obtaining the mRNA sequence, we can now determine the sequence of amino acids using the genetic code (codon table):
mRNA: GAA CGGU AUGC
Break it down into codons (groups of three nucleotides): GAA-CGG-UAG-C
According to the genetic code, GAA codes for glutamic acid (Glu), CGG codes for arginine (Arg), UAG is a stop codon, and C is the last base that does not form a complete codon.
So, the new sequence of amino acids is Glu-Arg. This differs from the original sequence, which would have encoded a different set of amino acids.
As for the suggestion of how this mutation may change the function of the new protein, it would depend on the specific protein being produced. Mutations in the DNA sequence can alter the amino acid sequence of the protein, which, in turn, can affect the protein's structure and function. In this case, the mutations resulted in the substitution of two amino acids. The functional impact of such mutations would depend on the specific properties and role of the original amino acids (methionine and phenylalanine) and the new amino acids (glutamic acid and arginine) in the protein's structure and function. To fully understand the implications of these mutations, further analysis of the specific protein and its role would be necessary.