if there is no colonies on the wild type or lysate or ADP-6only plates, did you obtain an approximate 10x-fold change in colony numbers between the different dilutions? did you expect this? pls help
Yes, expect that.
in the original plates, it is likely those colonies were dominated by one genotype. See this:
http://www.bio.fsu.edu/courses/mcb4403L/dilution.pdf
To determine if there is an approximate 10x-fold change in colony numbers between different dilutions, we need to compare the number of colonies in each dilution. If there are no colonies on any of the plates (wild type, lysate, and ADP-6 only), it implies that the number of colonies is zero.
In this case, since there are no colonies, we cannot calculate the fold change because we don't have any values to compare. The concept of fold change refers to the ratio between two values, so we need a non-zero value to calculate it.
Regarding expectations, it depends on the experiment or purpose of the dilution. If the expectation was to see visible growth and obtain colony numbers, then not obtaining any colonies indicates that something might have gone wrong. It could be due to various factors such as ineffective dilution, improperly prepared plates or media, or a problem with the sample itself.
To troubleshoot the issue, I would suggest reviewing the protocol and ensuring that the dilutions were performed correctly. It is also important to consider factors such as temperature, time, and culture conditions that might affect the growth of colonies.
If you need more specific guidance or clarification, please provide more details about the experiment or let me know.