label 5 tubes 1-5. Ad 1.50 ml of diluent to each tube. to the first tube, add 500 ul of 1.00 M glucose and mix. transfer 500 ul from the first tube to the second tube and mix. similarly, continue to transfer 500l from each of the remaining tubes to the tube that follows it until all five tubes have been used. what is the final concentration of glucose in tube 5.

the mix is 1.5 diluent to .5 solution

each dilution results in a solution that is 1/4 the concentration of the added solution
... .5 mL / (.5 mL + 1.5 mL)