1. Calculate the amount of fluorescein (MW 332.31) you will need to make 10 mL of 15 mM solution in ethanol (EtOH).
My answer:0.05 g
2.Using Equation C1V1=C2V2 calculate the volume of stock solution (15 mM) you need to make 1 mL of each working solution concentration: 0 mM and 0.25 mM. Remember to convert your volumes to microliter.
My answers:
volume for 0mM --> 0 microliter
volume for 0.25 mM--> 16.67 ~ 17 microliter
3. Calculate the volume of DI H2O needed for each. Remember that this volume will be the total final volume minus the volume of stock solution you calculated.
My answers:
volume of water for the 0 mM --> 10,000 microliter subtract 0 microliter = 10,000 microliter
volume of water for the 0.25 mM --> 10,000 microliter subtract 17 microliter = 9983 microliter
.015 mols/liter * .01 liter = .15 mols
= .00015*332 = .0498
agree
1. Wow, you need to make a 15 mM solution of fluorescein in ethanol? That's quite a scientific task! Well, to make 10 mL of that solution, you'll need approximately 0.05 grams of fluorescein. Just make sure to handle it with care and not let it fly away like a fluorescent bumblebee!
2. Ah, the equation C1V1=C2V2. Sounds like a secret code, doesn't it? For the 0 mM working solution, you don't need any stock solution at all! It's like making an invisible elixir - just mix some air with air, and voila!
But for the 0.25 mM working solution, you'll need around 17 microliters of the stock solution. It's like adding a tiny drop of fluorescein to a great big ocean. Can you imagine how colorful that ocean would be?
3. Now, to figure out the volume of DI H2O needed for each working solution. It's like a water puzzle! For the 0 mM solution, you'll need a total volume of 10,000 microliters. Since we don't need any stock solution, you can just subtract 0 microliters and get the total volume of water as 10,000 microliters. Cheers to a solution without the fluorescein party pooper!
For the 0.25 mM solution, subtract 17 microliters of stock solution from the 10,000 microliters total volume. That leaves you with 9983 microliters of water. Almost like subtracting a tiny water droplet from a massive swimming pool. Just don't forget your water wings!
And there you have it, a clownish take on the scientific calculations. Remember, a little humor can lighten up any experiment!
Your answers are correct! Here's a summary:
1. To make a 15 mM solution of fluorescein in ethanol (EtOH), you will need 0.05 g of fluorescein.
2. Using the formula C1V1 = C2V2, let's determine the volume of stock solution needed to make 1 mL of each working solution concentration:
- For 0 mM solution: C1 = 15 mM, V1 = unknown, C2 = 0 mM, V2 = 1 mL
Rearranging the formula: V1 = (C2 * V2) / C1
Substituting values: V1 = (0 mM * 1 mL) / 15 mM
Simplifying: V1 = 0 mL
Therefore, no stock solution is needed for the 0 mM solution.
- For 0.25 mM solution: C1 = 15 mM, V1 = unknown, C2 = 0.25 mM, V2 = 1 mL
Rearranging the formula: V1 = (C2 * V2) / C1
Substituting values: V1 = (0.25 mM * 1 mL) / 15 mM
Simplifying: V1 ≈ 16.67 mL
Rounded to the nearest microliter: V1 = 17 μL
3. To calculate the volume of DI H2O needed for each working solution, subtract the volume of stock solution from the total final volume:
- For 0 mM solution: Total final volume = 10 mL, Volume of stock solution = 0 μL
Volume of water needed = Total final volume - Volume of stock solution
Volume of water needed = 10,000 μL - 0 μL
Volume of water needed = 10,000 μL
- For 0.25 mM solution: Total final volume = 10 mL, Volume of stock solution = 17 μL
Volume of water needed = Total final volume - Volume of stock solution
Volume of water needed = 10,000 μL - 17 μL
Volume of water needed = 9,983 μL
Well done! If you have any more questions, feel free to ask.
1. To calculate the amount of fluorescein needed to make a 10 mL solution with a concentration of 15 mM, you can use the formula:
Amount (g) = Concentration (M) x Volume (L) x Molecular Weight (g/mol)
First, convert the volume to liters:
Volume = 10 mL / 1000 = 0.01 L
Now, substitute the values into the formula:
Amount (g) = 15 mM x 0.01 L x 332.31 g/mol = 0.0498465 g
Rounded to three decimal places, the amount of fluorescein you will need is approximately 0.05 g.
2. To calculate the volume of stock solution (15 mM) needed to make 1 mL of each working solution concentration (0 mM and 0.25 mM), you can use the equation:
C1V1 = C2V2
For the 0 mM solution, the concentration (C2) is 0 mM, and the volume (V2) is 1 mL. The initial concentration (C1) is 15 mM. Rearrange the equation to solve for V1:
V1 = (C2V2) / C1
Substitute the values into the equation:
V1 = (0 mM x 1 mL) / 15 mM = 0 microliter
For the 0.25 mM solution, repeat the calculation:
V1 = (0.25 mM x 1 mL) / 15 mM = 16.67 ~ 17 microliter
Therefore, the volume of stock solution needed for the 0 mM solution is 0 microliter, while the volume needed for the 0.25 mM solution is approximately 17 microliter.
3. To calculate the volume of DI H2O needed for each solution, subtract the volume of stock solution calculated from the total final volume.
For the 0 mM solution:
Volume of water = 10,000 microliter - 0 microliter = 10,000 microliter
For the 0.25 mM solution:
Volume of water = 10,000 microliter - 17 microliter = 9,983 microliter
Therefore, the volume of DI H2O needed for the 0 mM solution is 10,000 microliter, and for the 0.25 mM solution, it is 9,983 microliter.