You set up 5 samples that contained a range of sucrase concentrations but the same amount of
substrate (sucrose).
- The pH was kept constant in all tubes buy using the same amount of buffer.
- The temperature was kept constant in all tubes (room temp)
- A negative control was used (without the substrate sucrose) to prove that this reaction is due to enzyme activity. It is important to establish this, because sucrose can break down on its own to yield glucose and fructose but this reaction would take much longer without enzyme.
- There was no quantitative data, but a color key was used to distinguish between maximum, medium and low enzyme activity.
- By graphing the qualitative data, you observed that the reaction increases as the enzyme concentration is increased. This means, the more enzyme, the more sucrose can be broken down, the more products can be formed.
- However this effect can be observed up to a limit.
As stated, the results as evidenced in the experiment can only be observed up to a limit. Why?
A) A buildup of sugars alters the pH of the environment.
Eliminate
B) The products, glucose and fructose, denature the sucrase.
C) You eventually run out of sucrose to act as the substrate.
D) The reaction causes a change in temperature, that denatures the sucrase
It's C) You eventually run out of sucrose to act as the substrate.
You eventually run out of sucrose to act as the substrate. You must have both substrate and enzyme for the reaction to continue.
Thanks! This really helped, C is in fact correct.
it's c, get that right answer ma'am or sir
its c
The correct answer is C) You eventually run out of sucrose to act as the substrate.
In this experiment, the sucrase enzyme is responsible for breaking down sucrose into glucose and fructose. The more sucrase enzyme present, the more sucrose can be broken down, leading to the formation of more products.
However, this effect can only be observed up to a limit because eventually, you will run out of sucrose to act as the substrate for the enzyme. Once all the available sucrose molecules have been converted, the reaction cannot continue and there will be no further increase in enzyme activity.
This is different from option A, where a buildup of sugars altering the pH of the environment, because the pH was kept constant using a buffer. It is also different from option B, where the products glucose and fructose denature the sucrase, because denaturation typically occurs at extreme temperatures or pH levels, which were kept constant in this experiment.
Option D, where the reaction causes a change in temperature that denatures the sucrase, is also incorrect because the temperature was kept constant.
Therefore, the correct answer is C) You eventually run out of sucrose to act as the substrate.