1) Which tube will be most 'yellow' at the end of the experiment? ( For Enzyme Kinetics Experiment)
a) Substrate Concentration: 0.006M (my answer)
Inhibitor: absent
b) Substrate Concentration: 0.006M
Inhibitor: un-competitive inhibitor present
c) Substrate Concentration: 0.0005M
Inhibitor: un-competitive inhibitor present
d) Substrate Concentration: 0.0005M
Inhibitor: absent
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To determine which tube will be the most "yellow" at the end of the experiment for the Enzyme Kinetics Experiment, we need to understand the conditions of the experiment and how they affect the color change.
In this experiment, the substrate concentration and the presence of an inhibitor are the key factors that influence the color change. The higher the substrate concentration, the more yellow the tube will appear due to the reaction between the enzyme and substrate.
Now, let's analyze each option:
a) Substrate Concentration: 0.006M (my answer)
Inhibitor: absent
In this case, the substrate concentration is relatively high. Since there is no inhibitor present, the reaction between the enzyme and substrate can proceed without hindrance. Therefore, this tube is likely to be the most yellow at the end of the experiment.
b) Substrate Concentration: 0.006M
Inhibitor: uncompetitive inhibitor present
With the same substrate concentration as in option a, the presence of an uncompetitive inhibitor can potentially inhibit the enzyme's activity. This inhibition may result in a less intense yellow color compared to option a.
c) Substrate Concentration: 0.0005M
Inhibitor: uncompetitive inhibitor present
In this case, the substrate concentration is lower than in options a and b. With the presence of an uncompetitive inhibitor, the enzyme's activity is further inhibited. As a result, the color change may be less pronounced compared to the previous options.
d) Substrate Concentration: 0.0005M
Inhibitor: absent
Similar to option c, the substrate concentration is low, and there is no inhibitor present. However, due to the lower concentration of substrate, the reaction between the enzyme and substrate may be slower, resulting in a potentially weaker yellow color compared to option a.
Based on this analysis, option a (Substrate Concentration: 0.006M, Inhibitor: absent) is most likely to be the tube that appears the most yellow at the end of the experiment.