What is the mobile phase in this experiment?
A) 10% 6M NaOH and 90% acetone B) 10% 6M HCl and 90% acetone C) 10% 5M KCl and 90% methanol D) 10% 5M KCl and 90% acetone
Gel electrophoresis is an example of which type of chromatography?
A) Column chromatography B) Gas chromatography C) Paper chromatography D) Thin-layer chromatography
Which pair of words best describes chromatography?
A) Time-Writing B) Color-Writing C) Sleep-Walking D) Time-Traveling
In the event you need to write on your chromatogram, which should you use? A) Pen B) Pencil C) Marker D) Paintbrush
In this experiment the stationary phase is made out of what?
A) Polycarbonate B) Acetone C) Cellulose D) Glass beads
Fill in the blank: The more highly charged the complex, the the affinity for the more polar stationary phase and the the ions move?
A) greater, slower B) smaller, slower C) greater, faster D) smaller, faster
Fill in the blank: The paper in the paper chromatography lab is made of cellulose which has a surface covered in groups.
A) -OH B) -CH3 C) -NH D) -NH2
What is the ability for a liquid to flow in a narrow channel or narrow tube often against gravity, without the assistance of external forces.
A) viscosity B) capillary action C) deliquescence D) spectroscopy E) Tyndall effect
answers
To determine the mobile phase in this experiment, you will need to look at the options provided and identify the composition of the solvent mixture. Let's go through each option:
A) 10% 6M NaOH and 90% acetone
B) 10% 6M HCl and 90% acetone
C) 10% 5M KCl and 90% methanol
D) 10% 5M KCl and 90% acetone
Based on the options, the correct answer for the mobile phase in this experiment would be Option D, where the mobile phase consists of 10% 5M KCl and 90% acetone.
Now, let's move on to the next question regarding gel electrophoresis. Gel electrophoresis is an analytical technique used to separate DNA, RNA, or protein molecules based on their size and charge. To identify which type of chromatography gel electrophoresis falls under, you can look at the options:
A) Column chromatography
B) Gas chromatography
C) Paper chromatography
D) Thin-layer chromatography
Based on the options, the correct answer for the type of chromatography that gel electrophoresis belongs to is not listed. Gel electrophoresis is a specific technique that does not fit under the provided options, so the correct answer would be None of the above.
Moving on to the next question about describing chromatography, let's evaluate the given options:
A) Time-Writing
B) Color-Writing
C) Sleep-Walking
D) Time-Traveling
Among the options, the pair of words that best describes chromatography would be B) Color-Writing. Chromatography involves separating and identifying components of a mixture based on their colors and patterns formed during the separation process.
For the question regarding writing on the chromatogram, let's analyze the options:
A) Pen
B) Pencil
C) Marker
D) Paintbrush
When writing on a chromatogram, it is important to use an appropriate writing tool that will not interfere with the separation or react with the sample components. The correct option to use when writing on a chromatogram would be B) Pencil, as it does not contain any ink or other substances that could affect the separation or analysis.
Now, let's move on to the question about the stationary phase in the given experiment. Here are the options:
A) Polycarbonate
B) Acetone
C) Cellulose
D) Glass beads
To determine the stationary phase, we need to look for the material that remains stationary while the mobile phase moves through it. Among the options, the correct answer would be C) Cellulose. Cellulose is commonly used in paper chromatography as the stationary phase due to its ability to absorb and separate different components.
Next, we come to the question about the relationship between the charge of a complex and its affinity for the stationary phase in chromatography. Let's fill in the blank:
The more highly charged the complex, the _____ the affinity for the more polar stationary phase and the _____ the ions move?
A) greater, slower
B) smaller, slower
C) greater, faster
D) smaller, faster
In this case, the correct answer would be A) greater, slower. A more highly charged complex will have a stronger attraction to the polar stationary phase, resulting in slower movement of the ions in chromatography.
Moving on to the next question about the surface of the paper used in paper chromatography, let's complete the sentence:
"The paper in the paper chromatography lab is made of cellulose which has a surface covered in groups."
Among the options, the correct group found on the surface of cellulose is A) -OH, which represents hydroxyl groups. Cellulose, being a polysaccharide, contains multiple hydroxyl groups that play a role in the separation of components during paper chromatography.
Finally, let's address the question about the ability of a liquid to flow in a narrow channel or tube against gravity, without the assistance of external forces. The correct term for this phenomenon is:
A) viscosity
B) capillary action
C) deliquescence
D) spectroscopy
E) Tyndall effect
Among the provided options, the term that describes the ability of a liquid to flow in a narrow channel or tube against gravity without external forces is B) capillary action. Capillary action is a result of intermolecular forces and occurs due to the adhesive and cohesive properties of the liquid, allowing it to rise or flow upward in narrow spaces, such as capillary tubes or small channels.