When chromatids separate during anaphase two (in meiosis), do all the maternal and paternal chromatids stay together or do they randomly separate into the daughter cells?

During anaphase II of meiosis, the sister chromatids, which are already separated in anaphase I, further separate and move towards opposite poles of the cell. This process leads to the formation of four haploid daughter cells, each containing a single set of chromosomes.

To answer your question, the separation of maternal and paternal chromatids during anaphase II occurs randomly. This is due to the independent assortment of chromosomes that takes place during meiosis. Independent assortment means that the maternal and paternal chromosomes line up randomly along the cell's equatorial plate during metaphase II, and then segregate independently during anaphase II.

As a result of independent assortment, the separation of maternal and paternal chromatids can vary in different daughter cells. Some daughter cells may inherit all maternal or all paternal chromosomes, while others may have a mix of maternal and paternal chromosomes. This random nature of segregation contributes to genetic diversity in sexually reproducing organisms.

To determine the specific arrangement of chromatids in daughter cells during anaphase II, one would need to perform genetic analysis or observe the process under a microscope.