During your lab , you are provided with a stock antibody solution (Ab) that has a concentration of 400 ug/ul . you are provided with 10 ul( amount of stock antibody ) and 90 ul( amount of buffer ) . find the final dilution factor and the final Ab concentration.
What do u think?
Well, it seems like you're playing with some serious concentration here! Let's do some math, shall we?
We have 10 μl of stock antibody solution, which has a concentration of 400 μg/μl. Multiplying these two together, we get:
10 μl x 400 μg/μl = 4000 μg
Now, we need to determine the final dilution factor. Since we're adding 90 μl of buffer to our 10 μl of stock antibody, the total volume will be 100 μl.
The final dilution factor is calculated by dividing the total volume by the volume of the stock antibody solution. In this case, it will be:
100 μl / 10 μl = 10
So, the final dilution factor is 10.
To find the final antibody concentration, we divide the initial amount of antibody (4000 μg) by the final volume (100 μl):
4000 μg / 100 μl = 40 μg/μl
Therefore, the final antibody concentration is 40 μg/μl.
And there you have it! The final dilution factor is 10, and the final antibody concentration is 40 μg/μl. Happy diluting!
To find the final dilution factor and final antibody concentration, we need to calculate the total volume of the final solution.
The volume of the stock antibody solution provided is 10 μl, and the volume of the buffer added is 90 μl. Therefore, the total volume of the final solution is:
Total volume = Volume of stock antibody + Volume of buffer
Total volume = 10 μl + 90 μl
Total volume = 100 μl
Next, we can calculate the dilution factor, which is the ratio of the total volume to the volume of the stock antibody:
Dilution factor = Total volume / Volume of stock antibody
Dilution factor = 100 μl / 10 μl
Dilution factor = 10
The dilution factor is 10.
Finally, we can calculate the final concentration of the antibody in the solution using the dilution factor and the initial concentration of the stock antibody.
Initial concentration of stock antibody = 400 μg/μl
Final concentration = Initial concentration / Dilution factor
Final concentration = 400 μg/μl / 10
Final concentration = 40 μg/μl
Therefore, the final dilution factor is 10, and the final antibody concentration is 40 μg/μl.
To find the final dilution factor and the final antibody (Ab) concentration, we need to consider the original concentration of the stock antibody solution and the volumes of the stock antibody and buffer used in the dilution.
Given information:
- Concentration of the stock antibody solution (CStock) = 400 ug/ul
- Volume of the stock antibody used (VStock) = 10 ul
- Volume of the buffer used (VBuffer) = 90 ul
1. Calculate the diluted volume:
Diluted volume (VDiluted) = VStock + VBuffer
= 10 ul + 90 ul
= 100 ul
2. Calculate the final dilution factor:
Dilution factor = VDiluted / VStock
= 100 ul / 10 ul
= 10
3. Calculate the final antibody concentration:
Final Ab concentration (CFinal) = CStock / Dilution factor
= 400 ug/ul / 10
= 40 ug/ul
Therefore, the final dilution factor is 10, and the final antibody concentration is 40 ug/ul.