You have a green solution and you are trying to read the absorbance. However, when you use the spectrophotometer at 680mm, you get a reading of “HIGH”. You address this problem by diluting your samples
__Describe how & why you will make a 1/100 dilution
To make a 1/100 dilution of your green solution, you will need to mix 1 part of the solution with 99 parts of a suitable diluent.
Here's how you can do it:
1. Take a clean, dry container (such as a test tube or a beaker) and label it as "1/100 Dilution."
2. Measure 1 part of the green solution using a pipette or a graduated cylinder. The measurement can be in volume (e.g., milliliters) or in weight (e.g., grams), depending on the concentration units of your solution.
3. Transfer the measured volume of the green solution into the labeled container.
4. Then, add 99 parts of the diluent to the container. The diluent can be any suitable solvent or buffer that will not interfere with the absorbance reading.
5. Mix the solution thoroughly, either by gently swirling the container or by using a gentle vortex mixer. Ensure that the dilution is uniform throughout.
By making this 1/100 dilution, you are reducing the concentration of the green solution by a factor of 1/100. This dilution is necessary because the original undiluted solution produced a reading of "HIGH" on the spectrophotometer at 680nm.
The high absorbance reading suggests that the green solution is too concentrated for accurate measurement at this wavelength. Diluting the solution will decrease the concentration of the absorbing substance and bring the absorbance within the detectable range of the spectrophotometer. The 1/100 dilution factor is chosen based on the initial absorbance reading and the sensitivity range of the instrument.