Calculate the molar extinction coefficients for carotene and chlorophyll using the peaks of highest absorption and Beer’s law.Show your calculations.basically solve for e
A = ebc
Carotene (Yellow) = Highest peak 450 nm and 0.455 absorption
A = ebc
0.455 = e*1cm*c?
0.455/(1cm * c?)=e
e= ?
I know i'm suppose to find e but what is C in this equation. I know I did an IR or something at got a Carotene (Yellow) = Highest peak 450 nm and 0.455 absorption.
Thank You
You don't have the information here. It must be is another part of your experiment.
In order to calculate the molar extinction coefficient (e) using Beer's law, you need to know the concentration (c) of the substance being measured. The equation A = ebc relates the absorbance (A), the molar extinction coefficient (e), the path length (b), and the concentration (c) of the absorbing species.
In your case, you mentioned that the absorbance (A) for carotene at its highest peak (450 nm) is 0.455. To solve for the molar extinction coefficient (e), you need to know the concentration (c) of carotene.
If you have conducted an experiment to measure the absorbance, you should also have determined the concentration of carotene in your sample. The concentration value is usually given in units such as moles per liter (mol/L) or millimoles per liter (mmol/L). Let's assume you have a concentration value, let's say "c" in mmol/L.
Using the equation A = ebc, you can rearrange it to solve for e:
e = A / (bc)
Substituting the values you have:
e = 0.455 / (1 cm * c?)
Now, if you know the concentration value (c), you can substitute it into the equation to calculate the molar extinction coefficient (e) for carotene. Remember to include the correct unit for concentration (e.g., mmol/L) in your calculation.
Please note that Carotene and chlorophyll can have multiple absorption peaks, so it's important to consider the specific peak you are referring to in your calculation.