How would you make a serial dilution if you have a starting value 100mM of X
The are the values of X show different dilutions. I think she wants to know starting from 100mM of X how you would get the X values through a serial dilution.
X
0
1
3
1
10
30
100
If you could help me out by start me off because I am kind of confused with the question. Help is appreciated.
Here is something to remember: If you want to dilute something ten times: add nine parts water to one part original.
If you want to diltue something by a factor of XXX, take (XXX-1) parts of solvent, add one part original.
If you memorize this technique, it saves a lot of crazy calculations each time you have to dilute.
I am uncertain to the meaning of the table of values you typed, the formatting came out weird.
To perform a serial dilution starting from a 100mM solution of X, you would typically dilute the solution multiple times to obtain different concentrations of X.
Here's a step-by-step guide on how to perform a serial dilution:
1. Prepare a series of dilution tubes or containers. Label them accordingly with the desired dilution factors or concentrations you want to achieve for X.
2. Start with the 100mM solution of X in a separate container called the "stock solution."
3. To make the first dilution, take 1 part of the stock solution (e.g., 1 mL) and add it to 9 parts of the diluent (e.g., 9 mL of water). This corresponds to a 1:10 dilution (or a tenfold dilution).
4. Mix the solution thoroughly to ensure proper mixing and dilution.
5. The new concentration after the first dilution will be 1/10th (or 10%) of the original concentration, which is 10mM. Label this container as such.
6. To continue the serial dilution, take 1 part of the 10mM solution and add it to 9 parts of the diluent. This will result in a further 1:10 dilution.
7. Repeat the dilution process as needed to achieve the desired concentrations in the table you provided.
For example, to obtain a 3mM concentration of X, you would mix 1 part of the 30mM solution with 9 parts of the diluent. To obtain a 0.1mM concentration, you would mix 1 part of the 1mM solution with 9 parts of the diluent.
Remember, for each dilution, take the desired volume of stock solution and add it to the appropriate volume of diluent according to the specific dilution factor required. Also, ensure thorough mixing after each dilution to ensure an accurate and uniform distribution.
I hope this explanation helps you understand how to perform a serial dilution. If you need any further clarification, feel free to ask!