-----Chromatograph-----
Carrying out TLC on colorless compounds. Give two examples of methods to use in order to visualize the spots on the plates.
Carrying out TLC with a mixture of two compounds A and B. A is less polar than B. on a reverse phase plate which compound would be expected to have the larger Rf?
Confused with these two questions!
Thank you so much!!!!!!!
One example of #1 is amino acids. They are colorless so you don't see the spots until sprayed with a solution of ninhydrin. Tht turns them a visible color.
A second example is any compound that fluoresces in uv light. Shining the light makes them visible. A third example is to incorporate a radioactive tracer in the compound(s) and track the "spots" with a Geiger counter or a scintillation counter.
I am unfamiliar with reverse phase plate chromatography so I'll that one stand.