Molecular Bio

You are planning to digest human genomic DNA with the EcoRI restriction enzyme but realize you do not have any 10X buffer available to add to the reaction. The components of the 10X buffer are: 50 mM NaCl, 100 mM Tris, 10 mM MgCl2, and 0.025 % TritonX-100. You search around the lab and find the following solutions: 5 M NaCl, 1 M Tris, 0.5 % w/v TritonX-100. For MgCl2, you can only find it in powder form. The molecular weight for MgCl2 it is 203.30. Calculate how much of each stock solution, the amount of MgCl2 powder, and how much H2O you would use to make up 100 ml of a 10X solution (assume the MgCl2 powder does not alter the volume). Your answer should clearly indicate how you derived each number. State your volumes in milliliters and milligrams

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asked by D.
  1. 1 ml
    10 ml
    5 ml

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    posted by Jasmine

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